Pancreatic Polypeptide Polyklonaler Antikörper

Pancreatic Polypeptide Polyklonal Antikörper für IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15493-1-PBS

Synonyme

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  IHC staining of mouse pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  IF Staining of rat pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP, green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet. This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP) (green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet. This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 15493-1-AP (same clone as 15493-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Pancreatic Polypeptide (15493-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15493-1-PBS bindet in IHC, IF-P, FC (Intra), Indirect ELISA Pancreatic Polypeptide und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pancreatic Polypeptide fusion protein Ag7886
• Vollständiger Name: pancreatic polypeptide
• Berechnetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC032225
• Gene symbol: Pancreatic Polypeptide
• Gene ID (NCBI): 5539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.