- Featured Product
- KD/KO Validated
PARK2/Parkin Monoklonaler Antikörper
PARK2/Parkin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus und mehr (2)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
2H5A7
Kat-Nr. : 66674-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, Maushirngewebe, SH-SY5Y-Zellen, U-251-Zellen |
| Erfolgreiche Detektion in IHC | Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | RAW 264.7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 53 publications below |
| IHC | See 6 publications below |
| IF | See 30 publications below |
Produktinformation
66674-1-Ig bindet in WB, IHC, IF/ICC, ELISA PARK2/Parkin und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | PARK2/Parkin fusion protein Ag5179 |
| Vollständiger Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
| Berechnetes Molekulargewicht | 52 kDa |
| Beobachtetes Molekulargewicht | 42-52 kDa |
| GenBank-Zugangsnummer | BC022014 |
| Gene symbol | Parkin |
| Gene ID (NCBI) | 5071 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Parkin, a RING-type E3 ubiquitin-protein ligase, is involved in the ubiquitination pathway and contributes to protection from neurotoxicity induced by unfolded protein stresses. Its ubiquitin-protein ligase activity promotes the degradation of a viariety of proteins including itself. Mutations in Parkin are implicated in the pathogenesis of autosomal recessive familial Parkinson's disease. It has 8 isoforms produced by alternative splicing.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for PARK2/Parkin antibody 66674-1-Ig | Protokoll herunterladen |
| IHC protocol for PARK2/Parkin antibody 66674-1-Ig | Protokoll herunterladenl |
| IF protocol for PARK2/Parkin antibody 66674-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Autophagy CircEPS15, as a sponge of MIR24-3p ameliorates neuronal damage in Parkinson disease through boosting PINK1-PRKN-mediated mitophagy | ||
Nat Commun Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates. | ||
Oxid Med Cell Longev Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway. | ||
Oxid Med Cell Longev Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue | ||
Cell Death Discov Stomatin-like protein 2 deficiency exacerbates adverse cardiac remodeling |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paula (Verified Customer) (07-26-2024) | Worked well for Western Blotting when made fresh. However, after freezing antibody stock (in 3% BSA) did not work.
![]() |
FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
|







