Parvalbumin Polyklonaler Antikörper

Parvalbumin Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 29312-1-AP

Synonyme

PVALB, Parvalbumin alpha

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  WB analysis using 29312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29312-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum tissue using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

29312-1-AP bindet in WB, IHC, IF-P, ELISA Parvalbumin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Parvalbumin fusion protein Ag30189
• Vollständiger Name: parvalbumin
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: NM_002854
• Gene symbol: Parvalbumin
• Gene ID (NCBI): 5816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Front Mol Neurosci; PGC-1α regulates critical period onset/closure, mediating cortical plasticity; Authors - Wei-Jun Zhang
rat	IF	Physiol Res; High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats; Authors - X Zhang
rat	IHC	Transl Androl Urol; Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats; Authors - Chengyu Zhou
mouse	WB	Mol Neurobiol; Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation; Authors - Yu-Zhu Gao
mouse	IF	Cell Rep Methods; A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution; Authors - Jingtan Zhu
mouse	IF	STAR Protoc; Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID; Authors - Jingtan Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Parvalbumin (red) antibody worked in my human brain cortex sections, although it marks mostly the synaptic terminals more than the soma of the parvalbumin cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Parvalbumin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29312-1-AP)