Parvalbumin Polyklonaler Antikörper

Parvalbumin Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29312-1-PBS

Synonyme

PVALB, Parvalbumin alpha

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  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum tissue using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29312-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Parvalbumin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Parvalbumin fusion protein Ag30189
• Vollständiger Name: parvalbumin
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: NM_002854
• Gene symbol: Parvalbumin
• Gene ID (NCBI): 5816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).