Pentraxin 3 Polyklonaler Antikörper

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• KD/KO Validated

Pentraxin 3 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13797-1-PBS

Synonyme

PTX3, Pentaxin related protein PTX3, Pentaxin-related protein PTX3, Pentraxin related protein PTX3, Pentraxin-related protein PTX3

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  WB analysis of HepG2 using 13797-1-AP (same clone as 13797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13797-1-AP (same clone as 13797-1-PBS)

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13797-1-AP (same clone as 13797-1-PBS)

  HUVEC cells lysates were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13797-1-AP (same clone as 13797-1-PBS)

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells. This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13797-1-AP (same clone as 13797-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Pentraxin 3 antibody (13797-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13797-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13797-1-PBS bindet in WB, IF/ICC, Indirect ELISA Pentraxin 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pentraxin 3 fusion protein Ag4624
• Vollständiger Name: pentraxin-related gene, rapidly induced by IL-1 beta
• Berechnetes Molekulargewicht: 381 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC039733
• Gene symbol: Pentraxin 3/PTX3
• Gene ID (NCBI): 5806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif99, the pentraxin domain (PMID: 15771574). Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties (PMID: 15771574). Pentraxin 3 is an acute-phase glycoprotein that plays a role in regulating innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility, and cancer (PMID: 29412047). Pentraxin 3 is a biomarker for cardiovascular disease (PMID: 22347626). Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders (PMID: 22347626; 31057548). In WB, there're two major bands, an upper signal at 80-86 kDa and a lower signal at 42 kDa, representing Pentraxin 3 dimers and monomers, respectively (PMID: 18223257).