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Perforin Polyklonaler Antikörper

Perforin Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14580-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Perforin Polyclonal antibody (14580-1-AP)

    WB analysis using 14580-1-AP (same clone as 14580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14580-1-AP (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using Perforin Polyclonal antibody (14580-1-AP)

    WB analysis of mouse kidney using 14580-1-AP (same clone as 14580-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14580-1-AP (PRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14580-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14580-1-PBS bindet in WB, ELISA Perforin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Perforin fusion protein Ag6060
Vollständiger Name perforin 1 (pore forming protein)
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht 70-75 kDa
GenBank-ZugangsnummerBC063043
Gene symbol Perforin
Gene ID (NCBI) 5551
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)