Perforin Rekombinanter Antikörper

Perforin Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241009C10

Kat-Nr. : 83977-1-PBS

Synonyme

241009C10, Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein

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  WB analysis using 83977-1-RR (same clone as 83977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00872-1

  Cytometric bead array standard curve of MP00872-1, Perforin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83977-2-PBS. Detection antibody: 83977-1-PBS. Standard: Ag6060. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83977-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83977-1-PBS against Human Perforin was performed. The affinity constant is 1.33 nM.

• 

  Affinity and Kinetic Characterization of 83977-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83977-1-RR against Human Perforin were performed. The affinity constant is 1.05 nM.

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Geprüfte Anwendungen

Produktinformation

83977-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA Perforin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Perforin fusion protein Ag6060
• Vollständiger Name: perforin 1 (pore forming protein)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC063043
• Gene symbol: Perforin
• Gene ID (NCBI): 5551
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)