Pericentrin Polyklonaler Antikörper

Pericentrin Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 22271-1-AP

Synonyme

PCNT, Pericentrin-B, Pericentrin B

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  IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 22271-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 22271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen, C2C12-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

22271-1-AP bindet in IHC, IF/ICC, ELISA Pericentrin und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pericentrin
• Berechnetes Molekulargewicht: 378 kDa
• GenBank-Zugangsnummer: NM_006031
• Gene symbol: Pericentrin
• Gene ID (NCBI): 5116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.

Publikationen

Species	Application	Title
human	IF	Elife; De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.; Authors - Won-Jing Wang
human	IF	Cell Death Differ; Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis; Authors - Haiyu Song
human	IF	Cell Rep; Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation; Authors - Zhenzhou Fang
mouse	IF	Sci Rep; Muscarinic acetylcholine receptor 3 localized to primary endothelial cilia regulates blood pressure and cognition; Authors - Hannah C Saternos