Pericentrin Polyklonaler Antikörper

Pericentrin Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22271-1-PBS

Synonyme

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  IHC staining of human intrahepatic cholangiocarcinoma using 22271-1-AP (same clone as 22271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22271-1-AP (Pericentrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22271-1-AP (same clone as 22271-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (22271-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22271-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22271-1-PBS bindet in IHC, IF/ICC, Indirect ELISA Pericentrin und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pericentrin
• Berechnetes Molekulargewicht: 378 kDa
• GenBank-Zugangsnummer: NM_006031
• Gene symbol: Pericentrin
• Gene ID (NCBI): 5116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.