Perilipin-2 Polyklonaler Antikörper

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• KD/KO Validated

Perilipin-2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15294-1-PBS

Synonyme

ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2

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  WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15294-1-AP (same clone as 15294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (Perilipin-2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 15294-1-AP (same clone as 15294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15294-1-AP (same clone as 15294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin-2 antibody (15294-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15294-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15294-1-PBS bindet in WB, IHC, IF/ICC, ELISA Perilipin-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Perilipin-2 fusion protein Ag7539
• Vollständiger Name: adipose differentiation-related protein
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC005127
• Gene symbol: Perilipin-2
• Gene ID (NCBI): 123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases. This antibody is not suitable for immunofluorescence staining of frozen sections.