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Perilipin-2 Rekombinanter Antikörper

Perilipin-2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

230274F4

Kat-Nr. : CL594-80362-2

Synonyme

ADFP, Adipophilin, Adipose differentiation-related protein, ADRP, ADRP/Perilipin 2

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Perilipin-2 Recombinant mo (CL594-80362-2)

    FC experiment of HepG2 using CL594-80362-2

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite®594 Perilipin-2 Recombinant Antibody (CL594-80362-2, Clone:230274F4)(red), or 0.8 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80362-2 bindet in FC (Intra) Perilipin-2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Perilipin-2 fusion protein Ag7539
Vollständiger Name adipose differentiation-related protein
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerBC005127
Gene symbol Perilipin-2
Gene ID (NCBI) 123
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.