Periostin Monoklonaler Antikörper

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Periostin Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11A3

Kat-Nr. : 66491-1-PBS

Synonyme

1A11A3, OSF 2, OSF2, OSF-2, Osteoblast specific factor 2

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  WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

  BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66491-1-Ig (same clone as 66491-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis using 66491-1-Ig (same clone as 66491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis using 66491-1-Ig (same clone as 66491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66491-1-Ig (same clone as 66491-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66491-1-Ig (same clone as 66491-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66491-1-PBS bindet in WB, IHC, IF-P, IF-Fro, ELISA Periostin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Periostin fusion protein Ag14487
• Vollständiger Name: periostin, osteoblast specific factor
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 85-90 kDa
• GenBank-Zugangsnummer: BC106710
• Gene symbol: Periostin
• Gene ID (NCBI): 10631
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).