Peripherin Monoklonaler Antikörper

Peripherin Monoklonal Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H10B3

Kat-Nr. : 66317-1-PBS

Synonyme

1H10B3, NEF4, PRPH

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  WB analysis using 66317-1-Ig (same clone as 66317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66317-1-Ig (same clone as 66317-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 66317-1-Ig (same clone as 66317-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 66317-1-Ig (same clone as 66317-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51128-2

  Cytometric bead array standard curve of MP51128-2, Peripherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66317-4-PBS. Detection antibody: 66317-1-PBS. Standard:Ag11116. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66317-1-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Peripherin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peripherin fusion protein Ag11116
• Vollständiger Name: peripherin
• Berechnetes Molekulargewicht: 470 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC032703
• Gene symbol: Peripherin
• Gene ID (NCBI): 5630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.