Phospho-ACC1 (Ser79) Rekombinanter Antikörper

Phospho-ACC1 (Ser79) Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240235G11

Kat-Nr. : 80281-2-RR

Synonyme

ACC1 (Ser79), ACC1 Ser79, Acetyl Coenzyme A Carboxylase (phospho S79), p ACC1 (Ser79), p-ACC1

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  WB analysis using 80281-2-RR

  Non-treated NIH/3T33 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80281-2-RR (Phospho-ACC1 (Ser79) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 80281-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-ACC1 (Ser79) antibody (80281-2-RR, Clone: 240235G11 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  FC experiment of HeLa using 80281-2-RR

  1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-ACC1 (Ser79) Recombinant antibody (80281-2-RR, Clone:240235G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit λ-Phosphatase behandelte NIH/3T3-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

80281-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-ACC1 (Ser79) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: acetyl-Coenzyme A carboxylase alpha
• Berechnetes Molekulargewicht: 2383 aa, 275 kDa
• Beobachtetes Molekulargewicht: 250-270 kDa
• GenBank-Zugangsnummer: BC137287
• Gene symbol: Acetyl-CoA Carboxylase 1
• Gene ID (NCBI): 31
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537)

Publikationen

Species	Application	Title
mouse	WB	Food Chem; Enhanced bioactivity of honeysuckle-Cassia seeds extracts through Lactobacillus acidophilus and Bacillus subtilis co-fermentation: Impact on alcoholic liver disease and gut microbiota; Authors - Mengtian Zhou