Phospho-AKT (Thr308) Rekombinanter Antikörper

Phospho-AKT (Thr308) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242063B2

Kat-Nr. : 81232-10-RR

Synonyme

AKT (Thr308), p-AKT, p-AKT (Thr308), p-AKT Thr308, p-AKT-Thr308

---

• 

  WB analysis using 81232-10-RR

  Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  WB analysis using 81232-10-RR

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  WB analysis using 81232-10-RR

  Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  FC experiment of HeLa using 81232-10-RR

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-AKT (Thr308) Recombinant antibody (81232-10-RR, Clone:242063B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Calyculin A behandelte HSC-T6-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81232-10-RR bindet in WB, FC (Intra), ELISA Phospho-AKT (Thr308) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)