Phospho-AKT (Thr308) Rekombinanter Antikörper

Phospho-AKT (Thr308) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241845F1

Kat-Nr. : 81232-8-RR

Synonyme

Akt (Thr308), AKT1 (phospho T308), p-Akt, p-Akt (Thr308), p-Akt-Thr308

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  WB analysis using 81232-8-RR

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81232-8-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  FC experiment of HeLa using 81232-8-RR

  1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-AKT (Thr308) Recombinant antibody (81232-8-RR, Clone:241845F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81232-8-RR bindet in WB, FC (Intra), ELISA Phospho-AKT (Thr308) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine kinase Akt, also known as protein kinase B (PKB), contributes to a broad range of cellular functions including cell survival, proliferation, gene expression and migration of cells of most lineages. AKT has a wide range of cellular substrates, and the oncogenicity of AKT arises from activation of both proliferative and anti-apoptotic signaling, thus making this kinase an attractive target for cancer therapy. Activation of mammalian AKT depends on its recruitment to the membrane through binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3) to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain and C-terminal regulatory domain, respectively. (PMID: 34740102, PMID: 29017516)