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Phospho-AKT (Thr308) Rekombinanter Antikörper

Phospho-AKT (Thr308) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241845F1

Kat-Nr. : CL594-81232-8

Synonyme

Akt (Thr308), AKT1 (phospho T308), p-Akt, p-Akt (Thr308), p-Akt-Thr308

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated Phospho-AKT (Thr308) Recom (CL594-81232-8)

    FC experiment of HEK-293 using CL594-81232-8

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite®594 Phospho-AKT (Thr308) Recombinant Antibody (CL594-81232-8, Clone:241845F1)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-81232-8 bindet in FC (Intra) Phospho-AKT (Thr308) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name v-akt murine thymoma viral oncogene homolog 1
Berechnetes Molekulargewicht 56 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine kinase Akt, also known as protein kinase B (PKB), contributes to a broad range of cellular functions including cell survival, proliferation, gene expression and migration of cells of most lineages. AKT has a wide range of cellular substrates, and the oncogenicity of AKT arises from activation of both proliferative and anti-apoptotic signaling, thus making this kinase an attractive target for cancer therapy. Activation of mammalian AKT depends on its recruitment to the membrane through binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3) to the PH domain of AKT, and subsequent phosphorylation at two key residues, Thr308 and Ser473, located at the catalytic domain and C-terminal regulatory domain, respectively. (PMID: 34740102, PMID: 29017516)