Phospho-AKT1 (Ser473) Rekombinanter Antikörper

Phospho-AKT1 (Ser473) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

2M10

Kat-Nr. : 80462-1-RR

Synonyme

AKT (Ser473), AKT1 (Ser473), p AKT, p AKT (Ser473), p AKT Ser473

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  WB analysis using 80462-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80462-1-RR

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT1 (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80462-1-RR

  Non-treated and IGF1 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80462-1-RR

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80462-1-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  FC experiment of NIH/3T3 using 80462-1-RR

  1X10^6 NIH/3T3 cells untreated (dashed line) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-AKT1 (Ser473) (80462-1-RR, Clone:2M10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, HEK-293-Zellen, HEK-293T-Zellen, mit IGF-1 behandelte HEK-293T-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): mit Calyculin A behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

80462-1-RR bindet in WB, FC (Intra), ELISA Phospho-AKT1 (Ser473) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: NM_005163
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80462-1-RR specifically recognizes AKT1 phosphorylated at Ser473.

Publikationen

Species	Application	Title
mouse	WB	Phytomedicine; Apigenin ameliorates inflamed ulcerative colitis by regulating mast cell degranulation via the PAMP-MRGPRX2 feedback loop; Authors - Yihan Huang
human	WB	ACS Omega; Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation.; Authors - Kun Wang
mouse	WB	Biol Pharm Bull; Dictamnine ameliorates DNFB-induced atopic dermatitis like skin lesions in mice by inhibiting M1 macrophage polarization and promoting autophagy; Authors - Yihan Huang
human	WB	Phytomedicine; Schisandrin B regulates mitochondrial dynamics via AKT1 activation and mitochondrial targeting to ameliorate renal ischemia-reperfusion injury; Authors - Changhong Xu
human	WB	Phytother Res; Quercetin Alleviates Chronic Urticaria by Negatively Regulating IgE-Mediated Mast Cell Activation Through CD300f; Authors - Chenrui Zhao