Phospho-ASK1 (Ser83) Rekombinanter Antikörper

Phospho-ASK1 (Ser83) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241532B3

Kat-Nr. : 80072-2-PBS

Synonyme

P ASK1 (Ser83), P-ASK1 (Ser83), Phos ASK1 (Ser83), Phos-ASK1 (Ser83), ASK1

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  WB analysis using 80072-2-RR (same clone as 80072-2-PBS)

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80072-2-RR (Phospho-ASK1 (Ser83) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a control. This data was developed using the same antibody clone with 80072-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 80072-2-RR (same clone as 80072-2-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ASK1 (Ser83) Recombinant antibody (80072-2-RR, Clone:241532B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80072-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80072-2-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-ASK1 (Ser83) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 5
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC054503
• Gene symbol: ASK1
• Gene ID (NCBI): 4217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)