Phospho-ASK1 (Ser83) Rekombinanter Antikörper

Phospho-ASK1 (Ser83) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241532B3

Kat-Nr. : 80072-2-RR

Synonyme

P ASK1 (Ser83), P-ASK1 (Ser83), Phos ASK1 (Ser83), Phos-ASK1 (Ser83), ASK1

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  WB analysis using 80072-2-RR

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80072-2-RR (Phospho-ASK1 (Ser83) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a control.

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  FC experiment of HEK-293 using 80072-2-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ASK1 (Ser83) Recombinant antibody (80072-2-RR, Clone:241532B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, λ phosphatase treated HEK-293 cells
• Erfolgreiche Detektion in FC (Intra): λ phosphatase treated HEK-293 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80072-2-RR bindet in WB, FC (Intra), ELISA Phospho-ASK1 (Ser83) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 5
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC054503
• Gene symbol: ASK1
• Gene ID (NCBI): 4217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)