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Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyklonaler Antikörper

Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28790-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal antib (28790-1-AP)

    WB analysis using 28790-1-AP (same clone as 28790-1-PBS)

    Non-treated NIH/3T3 cells and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28790-1-AP (Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28790-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28790-1-PBS bindet in WB, Indirect ELISA Phospho-ATF2 (Thr71)/ATF7 (Thr53) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name activating transcription factor 2
Berechnetes Molekulargewicht 209 aa, 23 kDa
Beobachtetes Molekulargewicht 60-70 kDa
GenBank-ZugangsnummerBC026175
Gene symbol ATF2
Gene ID (NCBI) 1386
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.