Phospho-ATG4B (Ser383) Rekombinanter Antikörper

Phospho-ATG4B (Ser383) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242927A2

Kat-Nr. : 85703-1-PBS

Synonyme

P ATG4B (Ser383), P-ATG4B (Ser383), Phos ATG4B (Ser383), Phos-ATG4B (Ser383), ATG4B

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  WB analysis using 85703-1-RR (same clone as 85703-1-PBS)

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85703-1-RR (Phospho-ATG4B (Ser383) antibody) blocked with BSA only, Phospho-ATG4B (Ser383) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85703-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85703-1-PBS bindet in WB, Indirect ELISA Phospho-ATG4B (Ser383) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-48 kDa
• GenBank-Zugangsnummer: BC000719
• Gene symbol: ATG4B
• Gene ID (NCBI): 23192
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)