Phospho-ATR (Ser428) Rekombinanter Antikörper

Phospho-ATR (Ser428) Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250988D3

Kat-Nr. : 86330-1-RR

Synonyme

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

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  WB analysis of HEK-293 using 86330-1-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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  IF Staining of HEK-293 using 86330-1-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86330-1-RR bindet in WB, IF/ICC, ELISA Phospho-ATR (Ser428) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ataxia telangiectasia and Rad3 related
• Berechnetes Molekulargewicht: 301 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_001184
• Gene symbol: ATR
• Gene ID (NCBI): 545
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)