Phospho-Beta Catenin (Ser37) Polyklonaler Antikörper

Phospho-Beta Catenin (Ser37) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 28776-1-AP

Synonyme

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser37), Phospho-CTNNB1 (Ser37), PRO2286, β-Catenin

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  WB analysis using 28776-1-AP

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28776-1-AP (Phospho-Beta Catenin (Ser37) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

28776-1-AP bindet in WB, ELISA Phospho-Beta Catenin (Ser37) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86-100 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Catenin(β-catenin), also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-catenin is a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis.Deregulation of β-catenin activity is associated with multiple diseases including cancers. The N-terminal (1-49 amino acids) of β-catenin is the key region for its stability and the degradation by ubiquitin-proteasome system, and phosphorylation of β-catenin at the N-terminal Ser33, Ser37, Thr41, and Ser45 promotes its ubiquitination and degradation(PMID: 31930681).

Publikationen

Species	Application	Title
human	WB	J Agric Food Chem; Ursolic Acid Suppresses Colorectal Cancer by Down-Regulation of Wnt/β-Catenin Signaling Pathway Activity; Authors - Hui Zhao
human	WB	Adv Sci (Weinh); Hepatocyte-Targeted Lipid Nanoparticle Delivery of HERC2 Plasmid Controls Drug-Induced Hepatotoxicity by Limiting β-Catenin-Regulated CYP2E1 Expression; Authors - Yunzhi Liu
human	WB	J Mol Histol; Activation of the WNT4/ β-catenin/FOXO1 pathway by PDK1 promotes cervical cancer metastasis and EMT process; Authors - Shidong Chen
human	WB	Front Pharmacol; Gut microbiota-derived metabolite phenylacetylglutamine inhibits the progression of prostate cancer by suppressing the Wnt/β-catenin signaling pathway; Authors - Jing Lv