Phospho-Beta Catenin (Ser675) Polyklonaler Antikörper

Phospho-Beta Catenin (Ser675) Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 28853-1-AP

Synonyme

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser675), PRO2286, β-Catenin

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  WB analysis using 28853-1-AP

  Non-treated HT-29 cells, phosphatase inhibitor and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28853-1-AP (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of MCF-7 using 28853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (28853-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of MCF-7 using 28853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (28853-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit λ-Phosphatase behandelte HT-29-Zellen
• Erfolgreiche Detektion in IF/ICC: λ phosphatase treated MCF-7 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

28853-1-AP bindet in WB, IHC, IF/ICC, ELISA Phospho-Beta Catenin (Ser675) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742).

Publikationen

Species	Application	Title
mouse	WB	Endocrinology; Casein kinase 1α regulates testosterone synthesis and testis development in adult mice; Authors - Hongzhou Guo
human	WB	FASEB J; Verteporfin attenuates trauma-induced heterotopic ossification of Achilles tendon by inhibiting osteogenesis and angiogenesis involving YAP/β-catenin signaling; Authors - Gang Luo
human	WB	Bone; Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway; Authors - Yuanxi Leng
bovine	WB	Cells; DPPA2/4 Promote the Pluripotency and Proliferation of Bovine Extended Pluripotent Stem Cells by Upregulating the PI3K/AKT/GSK3β/β-Catenin Signaling Pathway; Authors - Shu Fang
human	WB	Exp Mol Med; Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop; Authors - Sheng Yang
mouse	WB,IHC	Am J Chin Med; Astragaloside IV Ameliorates Colonic Adenomatous Polyps Development by Orchestrating Gut Bifidobacterium and Serum Metabolome; Authors - Lu-Ping Wen