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Phospho-CDK1 (Tyr15) Rekombinanter Antikörper

Phospho-CDK1 (Tyr15) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251130A5

Kat-Nr. : 82751-5-PBS

Synonyme

cdc2 (Tyr15), CDK1 (Tyr15), p-cdc2, p-cdc2 (Tyr15), p-CDK1

Filter:
  • Western Blot (WB) analysis of HeLa cells using Phospho-CDK1 (Tyr15) Recombinant antibody (82751-5-RR)

    WB analysis of HeLa using 82751-5-RR (same clone as 82751-5-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 82751-5-RR (Phospho-CDK1 (Tyr15) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82751-5-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82751-5-PBS bindet in WB, Indirect ELISA Phospho-CDK1 (Tyr15) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name cell division cycle 2, G1 to S and G2 to M
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC014563
Gene symbol CDK1
Gene ID (NCBI) 983
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1. This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578)