Phospho-CHEK2 (Thr68) Polyklonaler Antikörper

Phospho-CHEK2 (Thr68) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 29012-1-AP

Synonyme

CDS1, CHEK2, CHK2, HuCds1, LFS2, PP1425, RAD53

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Galerie der Validierungsdaten

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  WB analysis using 29012-1-AP

  Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29012-1-AP (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:1000 incubated at room temperature for 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit MMS behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

29012-1-AP bindet in WB, ELISA Phospho-CHEK2 (Thr68) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CHK2 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC004207
• Gene symbol: CHEK2
• Gene ID (NCBI): 11200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)

Publikationen

Species	Application	Title
human	WB	Cell Prolif; eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.; Authors - Chao Mei
human	WB	Front Oncol; ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway; Authors - Xin Wen
human	WB	Research (Wash D C); Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer; Authors - Yangyang Li
human	WB	Cell Death Dis; PI3K/mTOR inhibitors promote G6PD autophagic degradation and exacerbate oxidative stress damage to radiosensitize small cell lung cancer; Authors - Huan Deng
human	WB	Front Oncol; Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway; Authors - Zhili Xia