Phospho-CHEK2 (Thr68) Rekombinanter Antikörper

Phospho-CHEK2 (Thr68) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1L2

Kat-Nr. : 81740-1-PBS

Synonyme

CHK2, CHEK2, EC:2.7.11.1, Cds1 homolog, CDS1

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  WB analysis of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

  Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81740-1-RR (same clone as 81740-1-PBS)

  Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 81740-1-RR (same clone as 81740-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.06 ug Phospho-CHEK2 (Thr68) Recombinant antibody (81740-1-RR, Clone:1L2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81740-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-CHEK2 (Thr68) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CHK2 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC004207
• Gene symbol: CHEK2
• Gene ID (NCBI): 11200
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)