• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-CHEK2 (Thr68) Rekombinanter Antikörper

Phospho-CHEK2 (Thr68) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1L2

Kat-Nr. : CL594-81740

Synonyme

CHEK2 (Thr68), p CHEK2, p CHEK2 (Thr68), p CHEK2 Thr68, p-CHEK2

Filter:
  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®594-conjugated Phospho-CHEK2 (Thr68) Reco (CL594-81740)

    FC experiment of PC-3 using CL594-81740

    1X10^6 PC-3 cells untreated (dashed lines) or treated with MMS which intracellularly stained with 0.25 ug Coralite®594 Phospho-Chek2 (Thr68) Recombinant Antibody (CL594-81740, Clone:1L2)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit MMS behandelte PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-81740 bindet in FC (Intra) Phospho-CHEK2 (Thr68) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name CHK2 checkpoint homolog (S. pombe)
Berechnetes Molekulargewicht 61 kDa
Beobachtetes Molekulargewicht65 kDa
GenBank-ZugangsnummerBC004207
Gene symbol CHEK2
Gene ID (NCBI) 11200
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746)