Phospho-CREB1 (Ser133) Rekombinanter Antikörper

Phospho-CREB1 (Ser133) Rekombinant Antikörper für WB, IHC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

5H5

Kat-Nr. : 81871-1-PBS

Synonyme

CREB1 (Ser133), p CREB1, p CREB1 (Ser133), p CREB1 Ser133, p-CREB1

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  WB analysis of HEK-293 using 81871-1-RR (same clone as 81871-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta-tubulin antibody as loading control. This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 81871-1-RR (same clone as 81871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 81871-1-RR (same clone as 81871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 81871-1-RR (same clone as 81871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HEK-293 using 81871-1-RR (same clone as 81871-1-PBS)

  Chromatin was prepared from HEK-293 cells treated with Forskolin (30 μM) for 1 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-CREB1 (Ser133) (81871-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 81871-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81871-1-PBS bindet in WB, IHC, Indirect ELISA, ChIP-qPCR Phospho-CREB1 (Ser133) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cAMP responsive element binding protein 1
• Berechnetes Molekulargewicht: 341 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-46 kDa
• GenBank-Zugangsnummer: BC010636
• Gene symbol: CREB1
• Gene ID (NCBI): 1385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)