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Phospho-CREB1 (Ser133) Rekombinanter Antikörper

Phospho-CREB1 (Ser133) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

5H5

Kat-Nr. : PE-81871

Synonyme

CREB1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using PE-conjugated Phospho-CREB1 (Ser133) Recombinant a (PE-81871)

    FC experiment of HEK-293 using PE-81871

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.125 ug PE Anti-Human Phospho-CREB1 (Ser133) (PE-81871, Clone:5H5), or 0.125 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-81871 bindet in FC (Intra) Phospho-CREB1 (Ser133) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name cAMP responsive element binding protein 1
Berechnetes Molekulargewicht 341 aa, 35 kDa
Beobachtetes Molekulargewicht 35-46 kDa
GenBank-ZugangsnummerBC010636
Gene symbol CREB1
Gene ID (NCBI) 1385
Konjugation PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)