Phospho-CREB1 (Ser133) Rekombinanter Antikörper
Phospho-CREB1 (Ser133) Rekombinant Antikörper für FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
FC (Intra)
Konjugation
PE Fluorescent Dye
CloneNo.
5H5
Kat-Nr. : PE-81871
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in FC (Intra) | Mit Calyculin A behandelte HEK-293-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
PE-81871 bindet in FC (Intra) Phospho-CREB1 (Ser133) und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | cAMP responsive element binding protein 1 |
Berechnetes Molekulargewicht | 341 aa, 35 kDa |
Beobachtetes Molekulargewicht | 35-46 kDa |
GenBank-Zugangsnummer | BC010636 |
Gene symbol | CREB1 |
Gene ID (NCBI) | 1385 |
Konjugation | PE Fluorescent Dye |
Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.09% sodium azide and 0.5% BSA |
Lagerungsbedingungen | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)