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Phospho-Cardiac Troponin I (Ser23/24) Rekombinanter Antikörper

Phospho-Cardiac Troponin I (Ser23/24) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241982B1

Kat-Nr. : 85002-1-PBS

Synonyme

cTnI, Cardiac troponin I, TNNI3, 241982B1

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Cardiac Troponin I (Ser23/24) Recombinant (85002-1-RR)

    WB analysis using 85002-1-RR (same clone as 85002-1-PBS)

    Non-treated mouse heart tissue, phosphatase inhibitor treated mouse heart tissue and λ phosphatase treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 85001-1-RR (Phospho-Cardiac Troponin I (Ser23/24) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85002-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85002-1-PBS bindet in WB, Indirect ELISA Phospho-Cardiac Troponin I (Ser23/24) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name troponin I type 3 (cardiac)
Berechnetes Molekulargewicht 210 aa, 24 kDa
Beobachtetes Molekulargewicht26 kDa
GenBank-ZugangsnummerBC096165
Gene symbol Cardiac Troponin I
Gene ID (NCBI) 7137
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)