Phospho-Cardiac Troponin I (Ser23/24) Rekombinanter Antikörper

Phospho-Cardiac Troponin I (Ser23/24) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241982B1

Kat-Nr. : 85002-1-RR

Synonyme

Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I (Ser23/24), P-Cardiac Troponin I, Phospho-Cardiac Troponin I, Phospho-Troponin I (Cardiac)

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  WB analysis using 85002-1-RR

  Non-treated mouse heart tissue, phosphatase inhibitor treated mouse heart tissue and λ phosphatase treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 85001-1-RR (Phospho-Cardiac Troponin I (Ser23/24) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, λ phosphatase treated mouse heart tissue

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85002-1-RR bindet in WB, ELISA Phospho-Cardiac Troponin I (Ser23/24) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: troponin I type 3 (cardiac)
• Berechnetes Molekulargewicht: 210 aa, 24 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC096165
• Gene symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)