Phospho-Caspase 9 (Thr125) Monoklonaler Antikörper

Phospho-Caspase 9 (Thr125) Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5E11

Rezensionen (1)

Kat-Nr. : 68136-1-Ig

Synonyme

CASP9, Caspase 9, Caspase 9/p35/p10, 1B5E11, APAF 3

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  WB analysis using 68136-1-Ig

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 68136-1-Ig (Phospho-Caspase 9 (Thr125) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte HSC-T6-Zellen, mit Calyculin A behandelte Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68136-1-Ig bindet in WB, ELISA Phospho-Caspase 9 (Thr125) und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 9, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002452
• Gene symbol: Caspase 9
• Gene ID (NCBI): 842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46 kDa precursor protein which can be cleaved into a 35 kDa subunit and a 11 kDa subunit. Control of all caspases is tightly regulated by a series of phosphorylation events enacted by several different kinases. Caspase-9 is the most heavily phosphorylated of all caspases, with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507, PMID: 29066624)

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Works on adult mouse retina embedded in paraffin as well as cryo embedded postnatal (p7) mouse tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Mouse postnatal (P7) retina/ Adult mouse retina