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Phospho-Chk1 (Ser317) Polyklonaler Antikörper

Phospho-Chk1 (Ser317) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28807-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Chk1 (Ser317) Polyclonal antibody (28807-1-AP)

    WB analysis using 28807-1-AP (same clone as 28807-1-PBS)

    Non-treated HEK-293T and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28807-1-AP (Phospho-Chk1 (Ser317) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28807-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28807-1-PBS bindet in WB, Indirect ELISA Phospho-Chk1 (Ser317) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name CHK1 checkpoint homolog (S. pombe)
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht55 kDa
GenBank-ZugangsnummerBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)