Phospho-CrkL (Tyr207) Rekombinanter Antikörper

Phospho-CrkL (Tyr207) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240881A2

Kat-Nr. : 84590-2-RR

Synonyme

CrkL (Tyr207), p CrkL, p CrkL (Tyr207), p-CrkL, p-CrkL (Tyr207)

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  WB analysis of NIH/3T3 using 84590-2-RR

  Non-treated NIH/3T3 cells and Blocked Phosphorylated peptide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-2-RR (Phospho-CrkL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Blocked Phosphorylated peptide treated NIH/3T3 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84590-2-RR bindet in WB, ELISA Phospho-CrkL (Tyr207) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• Berechnetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC043500
• Gene symbol: CRKL
• Gene ID (NCBI): 1399
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CrkL (v-crk avian sarcoma virus CT10 oncogene homolog-like) is a cytoplasmic adaptor protein that belongs to the Crk family of adaptors. Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)