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Phospho-EGFR (Tyr1016) Rekombinanter Antikörper

Phospho-EGFR (Tyr1016) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250687B2

Kat-Nr. : 82736-11-PBS

Synonyme

EGF Receptor (Tyr992), EGFR (Tyr1016), EGFR (Tyr992), p EGFR (Tyr1016), p-EGF Receptor (Tyr992)

Filter:
  • Western Blot (WB) analysis of A431 cells using Phospho-EGFR (Tyr1016) Recombinant antibody (82736-11-RR)

    WB analysis of A431 using 82736-11-RR (same clone as 82736-11-PBS)

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82736-11-RR (Phospho-EGFR (Tyr1016) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82736-11-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82736-11-PBS bindet in WB, Indirect ELISA Phospho-EGFR (Tyr1016) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Berechnetes Molekulargewicht 1210 aa, 134 kDa
Beobachtetes Molekulargewicht170 kDa
GenBank-ZugangsnummerBC094761
Gene symbol EGFR
Gene ID (NCBI) 1956
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) This antibody is equivalent to Tyr992.