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Phospho-EGFR (Tyr1069) Polyklonaler Antikörper

Phospho-EGFR (Tyr1069) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30277-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-EGFR (Tyr1069) Polyclonal antibody (30277-1-AP)

    WB analysis using 30277-1-AP (same clone as 30277-1-PBS)

    Non-treated HeLa and EGF treated HeLa cells were subjected to SDS PAGE followed by western blot with 30277-1-AP (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 30277-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30277-1-PBS bindet in WB, Indirect ELISA Phospho-EGFR (Tyr1069) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Berechnetes Molekulargewicht 1210 aa, 134 kDa
Beobachtetes Molekulargewicht170 kDa
GenBank-ZugangsnummerBC094761
Gene symbol EGFR
Gene ID (NCBI) 1956
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.