Phospho-EGFR (Tyr869) Rekombinanter Antikörper

Phospho-EGFR (Tyr869) Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241785G5

Kat-Nr. : 84839-1-RR

Synonyme

EGFR Tyr845, EGFR (Tyr845), p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845

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  WB analysis using 84839-1-RR

  Non-treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84839-1-RR (Phospho-EGFR (Tyr869) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

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  FC experiment of A431 using 84839-1-RR

  1x10^6 EGF treated A431 cells were intracellularly stained with 0.25 ug Phospho-EGFR (Tyr869) Recombinant Antibody (84839-1-RR, Clone:241785G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EGF treated A431 cells
• Erfolgreiche Detektion in FC (Intra): EGF treated A431 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84839-1-RR bindet in WB, FC (Intra), ELISA Phospho-EGFR (Tyr869) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 1210 aa, 134 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC094761
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) This antibody recognizes EGFR phosphorylation at tyrosine 869, which is also widely described as the tyrosine 845 site.