Phospho-EIF2S1 (Ser51) Polyklonaler Antikörper

Phospho-EIF2S1 (Ser51) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 28740-1-AP

Synonyme

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51

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Galerie der Validierungsdaten

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  WB analysis using 28740-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28740-1-AP (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:2000 incubated 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 3 publications below

Produktinformation

28740-1-AP bindet in WB, IHC, ELISA Phospho-EIF2S1 (Ser51) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_004094
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51

Publikationen

Species	Application	Title
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
mouse,human	WB,IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
mouse	WB	Oxid Med Cell Longev; The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.; Authors - Yichao Du
human	WB	Front Cardiovasc Med; Plasma Purification Treatment Relieves the Damage of Hyperlipidemia to PBMCs.; Authors - Xiao Meng Zhang
mouse	WB	J Mol Neurosci; Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.; Authors - Lei Chang
pig	WB	Microbiol Spectr; African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway; Authors - Han Zhong