Phospho-EIF2S1 (Ser51) Monoklonaler Antikörper

Phospho-EIF2S1 (Ser51) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1A4A11

Kat-Nr. : 68023-1-Ig

Synonyme

EIF2S1 (Ser51), p EIF2S1, p EIF2S1 (Ser51), p EIF2S1 Ser51, p-EIF2S1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, Mit Calyculin A behandelte HEK-293-Zellen, Mit Calyculin A behandelte HeLa-Zellen, mit Calyculin A behandelte HSC-T6-Zellen, mit Calyculin A behandelte NIH/3T3-Zellen, Mit Calyculin A behandelte PC-3-Zellen, HeLa-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCMit Calyculin A behandelte HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68023-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA Phospho-EIF2S1 (Ser51) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht36 kDa
GenBank-ZugangsnummerNM_004094
Gene symbol EIF2S1
Gene ID (NCBI) 1965
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Phospho-EIF2S1 (Ser51) antibody 68023-1-IgProtokoll herunterladen
IF protocol for Phospho-EIF2S1 (Ser51) antibody 68023-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Int Immunopharmacol

TRx0237 induces apoptosis and enhances anti-PD-1 immunotherapeutic efficacy in anaplastic thyroid Cancer

Authors - Qingyang Ning
humanWB

bioRxiv

Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells

Authors - Juan Jesus Vicente
humanWB

Cell Death Dis

AURKA emerges as a vulnerable target for KEAP1-deficient non-small cell lung cancer by activation of asparagine synthesis

Authors - Bing Deng
humanWB

J Transl Med

The microtubule targeting agent ST-401 triggers cell death in interphase and prevents the formation of polyploid giant cancer cells

Authors - Juan Jesus Vicente
humanWB

Environ Pollut

Copper exposure induces mitochondrial dysfunction and hepatotoxicity via the induction of oxidative stress and PERK/ATF4 -mediated endoplasmic reticulum stress

Authors - Meng Li
mouse,humanWB

Microbiol Res

Berberine ameliorates vascular dysfunction by downregulating TMAO-endoplasmic reticulum stress pathway via gut microbiota in hypertension

Authors - Zhichao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Morgane (Verified Customer) (09-15-2025)

Displays aspecific band and requires a bit of exposure using ECL and HRP-coupled secundary antibody but band is still visible

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HCT116
Phospho-EIF2S1 (Ser51) Antibody Western Blot validation (1/2000 dilution) in HCT116 (Cat no:68023-1-Ig)
FH

Alla (Verified Customer) (07-24-2025)

Very good antibody.Have good image,but was not ale to uploade it.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:12,000
  • Cell Tissue Type: HEK 293