Phospho-EIF2S1 (Ser51) Monoklonaler Antikörper

Phospho-EIF2S1 (Ser51) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4A11

Kat-Nr. : 68023-1-PBS

Synonyme

EIF2S1 (Ser51), p EIF2S1, p EIF2S1 (Ser51), p EIF2S1 Ser51, p-EIF2S1

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  WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (HRP-66009) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  WB analysis using 68023-1-Ig (same clone as 68023-1-PBS)

  Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (HRP-66240) antibody as loading control. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68023-1-Ig (same clone as 68023-1-PBS)

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68023-1-Ig (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68023-1-Ig (same clone as 68023-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-EIF2S1 (Ser51) antibody (68023-1-Ig, Clone: 1A4A11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 68023-1-Ig (same clone as 68023-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-EIF2S1 (Ser51) (68023-1-Ig, Clone:1A4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 68023-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50181-1

  Cytometric bead array in cell lysate using MP50181-1, Phospho-EIF2S1 (Ser51) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68479-1-PBS. Detection antibody: 68023-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Geprüfte Anwendungen

Produktinformation

68023-1-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Phospho-EIF2S1 (Ser51) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_004094
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.