Phospho-EIF2S1 (Ser51) Monoklonaler Antikörper

Phospho-EIF2S1 (Ser51) Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

1A4A11

Kat-Nr. : CL488-68023

Synonyme

EIF2S1, EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A

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  IF Staining of HeLa using CL488-68023

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-EIF2S1 (Ser51) antibody (CL488-68023, Clone: 1A4A11 ) at dilution of 1:200.

• 

  FC experiment of PC-3 using CL488-68023

  1x10^6 Calyculin A treated PC-3 cells were intracellularly stained with 0.13 ug CoraLite® Plus 488-conjugated Phospho-EIF2S1 (Ser51) Monoclonal antibody (CL488-68023, Clone:1A4A11)(red), or 0.13 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Mit Calyculin A behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-68023 bindet in IF/ICC, FC (Intra) Phospho-EIF2S1 (Ser51) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_004094
• Gene symbol: EIF2S1
• Gene ID (NCBI): 1965
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Publikationen

Species	Application	Title
	IF	Nat Commun; LNP-RNA-engineered adipose stem cells for accelerated diabetic wound healing; Authors - Yonger Xue