Phospho-EIF4E (Ser209) Rekombinanter Antikörper

Phospho-EIF4E (Ser209) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242049G10

Kat-Nr. : 84981-1-PBS

Synonyme

EIF4E, EIF4F, EIF4EL1, eIF-4E, eIF 4E

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  WB analysis of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

  Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

  1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR, Clone:242049G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84981-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-EIF4E (Ser209) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: eukaryotic translation initiation factor 4E
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC012611
• Gene symbol: EIF4E
• Gene ID (NCBI): 1977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)