Phospho-ERK1/2 (Thr202/Tyr204) Rekombinanter Antikörper

Phospho-ERK1/2 (Thr202/Tyr204) Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

8D12

Kat-Nr. : 80031-1-PBS

Synonyme

ERK1/2 (Thr202/Tyr204), p ERK1/2, p ERK1/2 (Thr202/Tyr204), p ERK1/2 Thr202/Tyr204, p-ERK1/2

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  WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 80031-1-RR (same clone as 80031-1-PBS)

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (ERK1/2-phospho-Tyr204 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 80031-1-RR (same clone as 80031-1-PBS)

  1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody (80031-1-RR, Clone:8D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80031-1-PBS bindet in WB, FC (Intra), Indirect ELISA Phospho-ERK1/2 (Thr202/Tyr204) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 3
• Berechnetes Molekulargewicht: 38-43 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: NM_002746
• Gene symbol: ERK1
• Gene ID (NCBI): 5595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.