Phospho-ERK1/2 (Thr202/Tyr204) Rekombinanter Antikörper
Phospho-ERK1/2 (Thr202/Tyr204) Rekombinant Antikörper für FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
FC (Intra)
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
8D12
Kat-Nr. : CL594-80031
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in FC (Intra) | mit Calyculin A behandelte HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-80031 bindet in FC (Intra) Phospho-ERK1/2 (Thr202/Tyr204) und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | mitogen-activated protein kinase 3 |
Berechnetes Molekulargewicht | 38-43 kDa |
Beobachtetes Molekulargewicht | 38-40 kDa |
GenBank-Zugangsnummer | NM_002746 |
Gene symbol | ERK1 |
Gene ID (NCBI) | 5595 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.