Phospho-ErbB3/HER3 (Tyr1289) Rekombinanter Antikörper

Phospho-ErbB3/HER3 (Tyr1289) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250208A12

Kat-Nr. : 85053-6-PBS

Synonyme

ErbB3/HER3 (Tyr1289), HER3/ErbB3 (Tyr1289), p ErbB3/HER3 (Tyr1289), p HER3/ErbB3, p-ErbB3/HER3 (Tyr1289)

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  WB analysis using 85053-6-RR (same clone as 85053-6-PBS)

  Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 85053-6-RR (Phospho-ErbB3/HER3 (Tyr1289) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85053-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85053-6-PBS bindet in WB, Indirect ELISA Phospho-ErbB3/HER3 (Tyr1289) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC002706
• Gene symbol: ERBB3
• Gene ID (NCBI): 2065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Unlike EGFR and HER2, their paralogue HER3 does not possess intrinsic kinase activity due to substitutions at critical positions in the kinase domain. To phosphorylate tyrosine residues in the HER3 cytoplasmic tail to recruit and activate intracellular signaling molecules, it must interact with another functional tyrosine kinase. HER3 exhibits this crosstalk preferentially with HER2 and EGFR, with which it can form heterodimers. Ligand-induced hetero-dimerization with EGFR or HER2 promotes HER3-tyrosine phosphorylation at 6 positions (1054, 1197, 1222, 1260, 1276, and 1289) in the C-terminal tail capable of recruiting of the SH2-domain-containing p85 activator of phosphatidyl-inositol-3 kinase (PI3K) and promoting activation of the downstream Akt signaling pathway. (PMID: 35249128)