Phospho-ErbB3/HER3 (Tyr1289) Rekombinanter Antikörper

Phospho-ErbB3/HER3 (Tyr1289) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250208A12

Kat-Nr. : 85053-6-RR

Synonyme

ErbB3/HER3 (Tyr1289), HER3/ErbB3 (Tyr1289), p ErbB3/HER3 (Tyr1289), p HER3/ErbB3, p-ErbB3/HER3 (Tyr1289)

---

• 

  WB analysis using 85053-6-RR

  Non-treated MCF-7 cells and human Neuregulin-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 85053-6-RR (Phospho-ErbB3/HER3 (Tyr1289) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, human Neuregulin-1 treated MCF-7 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85053-6-RR bindet in WB, ELISA Phospho-ErbB3/HER3 (Tyr1289) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC002706
• Gene symbol: ERBB3
• Gene ID (NCBI): 2065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Unlike EGFR and HER2, their paralogue HER3 does not possess intrinsic kinase activity due to substitutions at critical positions in the kinase domain. To phosphorylate tyrosine residues in the HER3 cytoplasmic tail to recruit and activate intracellular signaling molecules, it must interact with another functional tyrosine kinase. HER3 exhibits this crosstalk preferentially with HER2 and EGFR, with which it can form heterodimers. Ligand-induced hetero-dimerization with EGFR or HER2 promotes HER3-tyrosine phosphorylation at 6 positions (1054, 1197, 1222, 1260, 1276, and 1289) in the C-terminal tail capable of recruiting of the SH2-domain-containing p85 activator of phosphatidyl-inositol-3 kinase (PI3K) and promoting activation of the downstream Akt signaling pathway. (PMID: 35249128)