Phospho-Estrogen Receptor α (Ser118) Rekombinanter Antikörper

Phospho-Estrogen Receptor α (Ser118) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250278F8

Kat-Nr. : 84564-6-PBS

Synonyme

ESR1, ER, ER alpha, Era, ER-alpha

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  WB analysis using 84564-6-RR (same clone as 84564-6-PBS)

  T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84564-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84564-6-PBS bindet in WB, Indirect ELISA Phospho-Estrogen Receptor α (Ser118) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: estrogen receptor 1
• Berechnetes Molekulargewicht: 595 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC128573
• Gene symbol: ER
• Gene ID (NCBI): 2099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)