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Phospho-FOXO1 (Ser319) Rekombinanter Antikörper

Phospho-FOXO1 (Ser319) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250275C3

Kat-Nr. : 82997-7-PBS

Synonyme

FOXO1 (Ser319), p FOXO1 (Ser319), p-FOXO1, p-FOXO1 (Ser319), p-FOXO1(Ser319)

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-FOXO1 (Ser319) Recombinant antibody (82997-7-RR)

    WB analysis using 82997-7-RR (same clone as 82997-7-PBS)

    HEK-293T and MCF-7 cell lysates were subjected to SDS PAGE followed by western blot with 82997-7-RR (Phospho-FOXO1 (Ser319) antibody) blocked with BSA only, Phospho-FOXO1 (Ser319) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82997-7-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82997-7-PBS bindet in WB, Indirect ELISA Phospho-FOXO1 (Ser319) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name forkhead box O1
Berechnetes Molekulargewicht 655 aa, 70 kDa
Beobachtetes Molekulargewicht 70-80 kDa
GenBank-ZugangsnummerBC021981
Gene symbol FOXO1
Gene ID (NCBI) 2308
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)